Angiogenesis is the formation of new blood vessels from the existing vasculature. It is a
multistage process in which activated endothelial cells (EC) degrade basement membrane,
sprout from the parent vessel, migrate, proliferate, align, undergo tube formation, and
eventually branch and anastomose with adjacent vessels. Here we describe a three‐
dimensional in vitro assay that reproduces each of these steps. Human umbilical vein
endothelial cells (HUVEC) are cultured on microcarrier beads, which are then embedded in …