A plasma sample preparation for mass spectrometry using an automated workstation

Q Fu, CW Johnson, BK Wijayawardena… - JoVE (Journal of …, 2020 - jove.com
Sample preparation for mass spectrometry analysis in proteomics requires enzymatic
cleavage of proteins into a peptide mixture. This process involves numerous incubation and
liquid transfer steps in order to achieve denaturation, reduction, alkylation, and cleavage.
Adapting this workflow onto an automated workstation can increase efficiency and reduce
coefficients of variance, thereby providing more reliable data for statistical comparisons
between sample types. We previously described an automated proteomic sample …