(A) Heat map of PAQR11, ZEB1, and miR levels in KP cells (n = 3 samples per cell) with legend (left). (B) The PAQR11 3′-UTR and predicted miR binding sites are shown at top. Bar graphs show luciferase activities in 344SQ cells (left) and H1299 cells (right) transiently cotransfected with PAQR11 3′-UTR reporter and negative control (NC) or gene-specific pre-miRs (n = 3 samples per condition). ^§P < 0.0001.(C) Illustrations of PAQR11 3′-UTR reporters (top) showing the mutated miR-148a site (left), mutated miR-206 sites (middle), and mutated miR-200c sites (right). Bar graphs show luciferase activities in 344SQ cells transiently cotransfected with negative control (NC) or gene-specific pre-miRs and reporters that were empty (Vec) or contained WT PAQR11 3′-UTR sequences or PAQR11 3′-UTRs with mutations in either (MT1 or MT2) or both (MT1+2) of the predicted binding sites. (D) The bar graph shows the results of qPCR analysis of ectopic miR-148a, -206, and -200c levels in 393P_ZEB1 cells stably transfected with vectors expressing ectopic miRs or with empty vectors (Vec). (E) The bar graph (n = 3 samples per condition) shows the results of qPCR analysis of PAQR11 mRNA levels in 393P_ZEB1 cells stably transfected with vectors expressing ectopic miRs or with empty vectors (Vec). (F) Western blot analysis of PAQR11 levels in 344SQ cells (top) and H1299 cells (bottom) transfected with pre-miRs or negative control (NC) oligos. β-Actin was included as loading control. (G) The scatter plots show Golgi areas normalized to nuclear areas in 393P_ZEB1 cells (left), 344SQ cells (middle), and H1299 cells (right) that stably express ectopic miRs or empty vector (Vec). Each dot represents values from a single cell. P values were determined using 2-tailed Student’s t test or ANOVA for comparisons between 2 groups or more than 2 groups, respectively. Results were replicated (n ≥ 2 experiments). See complete unedited blots in the supplemental material.